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Description
RT-PCR An RNeasy kit (Qiagen, 74104) was used to extract total RNA from mice hippocampal tissue, and then the Transcriptor High Fidelity cDNA synthesis kit (Roche, 5081963001) was used to reverse transcribe the RNA into cDNA

Die Patches werden einfach auf die Haut geklebt, mglichst direkt auf die Stelle, wo das Peptid wirken soll

The peptide was allowed to denature over a 4 hour time period and 100 uL aliquots of the peptide solution was removed, mixed according to the above mentioned procedure and characterized
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Flint et al., 1993)
