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A positive result was defined as having a statistically significant difference in at least one concentration, compared to the concurrent vehicle control, along with an at least twofold increase in TI

Next, PCR enrichment of the adapter-ligated library was conducted (six cycles of PCR)

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Drugs that can cause megaloblastic anemia include: Antifolate drugs like methotrexate Anticonvulsants such as phenytoin Other medications that affect folate or vitamin B12 metabolism Pernicious Anemia: The Most Common Form of Megaloblastic Anemia Pernicious anemia is the most common type of megaloblastic anemia