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Images were quantified using ImageJ software, and the ratio of 510 to 590 nm was calculated

Acknowledgements This work was supported by grants from the National Natural Science Foundation of China (grant no

I work out but try not to push myself too hard so that I dont crash (like I used to)

Calreticulin, a Ca 2+ -binding chaperone, alleviates this effect by stabilizing ER Ca 2+ levels when overexpressed (Oyadomari et al

Method validation LOD and LOQ According to the guidelines for biological sample analysis 37 , the limit of detection (LOD) and limit of quantification (LOQ) are defined as the lowest concentrations giving the signal/noise ratio of 3 and 10, respectively