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This timeframe allows the new hair growing from the follicle to benefit from the improved internal antioxidant environment

Assembly of the three PCR products two from both flanking regions of the target gene, one from the antibiotic resistance gene was accomplished using overlap extension PCR with primers hybridizing at both extreme ends, and the resulting DNA fragment was purified by agarose gel electrophoresis

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For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays