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Description
& Laub, M
The supernatant was mixed with SDS sample buffer [50 mM Tris-HCl pH 6.8, 2% (w/v) SDS, 5% (w/v) glycerol, 0.02% (w/v) bromophenol blue, and 200 mM DTT] and heated at 70 C for 20 min
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