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Li et al., The notion that manipulating DNA methylation has an impact on MDD encouraged studies measuring DNA methylation levels as potential biomarkers to predict MDD and its severity in vulnerable populations as well as treatment outcomes

However, the small sample-size, the short treatment period, the absence of control groups, the lack of liver biopsy evaluation after treatment are just some of the limitations of these studies

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By interfering with DNA methylation and chromatin control, disruptions in piRNA synthesis or PIWI protein activity result in aberrant gametes and prevent appropriate zygotic genome activation