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Description
RNA samples were reverse transcribed using the Prime Script RT Reagent Kit (Perfect Real Time, Takara Bio Inc., Shiga, Japan)
2 mM 2-mercaptoethanol, 0.01% NP-40) at 4 C for 1 h
The GSH column was equilibrated with 5-column volume (CV) buffer containing 150 mM NaCl (Equil Buffer)
Digestion begins when you begin to chew and continues while the body is in a para-sympathetic (relaxed) state
Subsequently, we conducted domain-mapping analyses
