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Freshly dissected rat corpus cavernosum tissues were rinsed and vigorously agitated in ice-cold PBS to remove residual blood cells, followed by mincing and enzymatic digestion with 2.5 mg/ml collagenase type I, 4 mg/ml collagenase type IV, 0.1 mg/ml neutral protease, and 2 mg/ml DNase I at 37C for 40 min[31]

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