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Briefly, dorsal surface was shaved and a 10 x 3 cm caudally based dorsal skin flap was elevated from the scapulas to the iliac crest (Fig 1A), then sutured back into its original site with stainless steel wound clips

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Importantly, this inhibition occurred without bactericidal effects on gut microbiota, as confirmed by preserved alpha diversity and the persistence of TMA-producing genera such as Collinsella and Clostridium post-treatment

Bile is a substance which helps in digestion by emulsifying fat and cholesterol and allowing their absorption in the small intestine, and is produced in the liver and stored in the gall bladder