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The enzyme was dissolved in phosphate-buffered saline at pH 7.4, sterilized through a 0.22-m membrane, and administered under ultrasound guidance

Research Use Limitation Used solely for in vitro experiments Not permitted for clinical trials involving humans Not permitted for human or veterinary administration Not permitted for investigational human use Independently analysed by Liquilabs s.r.o

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The 3.0 mL fill gives ~3.33 mg/mL, so 1 unit 33.3 mcg on a U-100 syringe (150 mcg = 4.5 units, 600 mcg = 18 units)

Rohr, M