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Antioxidants Redox Signal 2011
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Specifically, after protein removal by centrifugation at 15,000g for 1 min a slight alkaline pH was restored adding 50 L of 2 M TRIS to 400 L of the acid supernatant
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The resulting stock hemolysate was diluted 1:500 and resuspended in 50 mM phosphate-buffered saline
