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Description
The GSH 2.5 -HA MN patches and 350 L GSH 2.5 -HA solution used for MN fabrications were applied on excised Sprague-Dawley (SD) rat skin (~2 mm thick) placed between the donor and receptor chambers in the Franz diffusion cell, respectively

[Crossref] Lin RT, Hsi E, Lin HF, Liao YC, Wang YS, et al

The effects are very sustainable, though reversible, i.e., the skin could gradually regress to its original colour when the supplementation and supportive habits are discontinued
doi: 10.3390/nu12030784 172

Disc herniations can develop gradually through repeated microtrauma and age-related degeneration, or they can occur suddenly from an acute injury