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The cells were incubated with primary anti-PD-L1 (1:200, A1645, ABclonal, RRID: AB_2763702) overnight at 4 C, followed by incubation with Cy3-labeled goat anti-rabbit IgG (1:500, A0516, Beyotime) for 60 min at RT in the dark

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Each dot represents an individual animal (males, squares

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