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The gradient was as follows: 0 min, 15% A
(Source.) And so, how do we best determine our Vitamin B12 status
For transfection, the cells were seeded in 6-well plates at 1 10 6 cells/well and allowed to grow overnight to 75% confluence
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Generally, I suggest 1 B12 shot weekly for 6 weeks to build it up in the system
