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In oncology, multi-omics analyses have successfully pinpointed epigenetically regulated genes that function as dependable biomarkers and therapeutic targets, underscoring the translational efficacy of these methodologies ( 5.3 Clinical translational research The clinical application of DNA methylation research in AS represents a nascent domain with considerable potential to enhance diagnosis, prognosis, and individualized treatment approaches ( To determine the diagnostic and prognostic value of methylation biomarkers in AS, large-scale clinical cohort validations are necessary