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[DOI] [PubMed] [Google Scholar] Morales A, Garca-Ruiz C, Miranda M, Mar M, Colell A, Ardite E, Fernndez-Checa JC

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To initiate the differentiation process, differentiation medium consisting of DMEM/F12 (Sigma-Aldrich) with 1% N2 supplement (Thermo Fisher Scientific), 1 g/mL tetracycline (Sigma-Aldrich), 0.49 mg/mL dibutyryl-cyclic adenosine monophosphate (Sigma-Aldrich), and 2 ng/L glial cell line-derived neurotrophic factor (R&D Systems) was used [53]