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Immediately after isolation, PBMC were seeded on a 96-well microplate at a density of 10 5 cells per well and incubated for 69.5 h at 37C and 5% CO 2 with or without stimulation of concanavalin A (ConA, 2.5 g/ml
The binary mixtures of megestrol acetate and 17alpha-ethynylestradiol adversely affect zebrafish reproduction
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In addition, AMPK and SIRT1 gene expressions increased in the RAD + LCAR group compared to the RAD group, while TGF-1 gene expression decreased
