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Then youll equilibrate your beads in the binding buffer, or the same buffer that youll load your GST-fusion protein onto the glutathione beads, as well discuss in the next section

After incubation, the sample was centrifuged at 4000 rpm for 5 min and the supernatant was discarded

In IDH -WT glioblastoma (GBM), tumor metabolism is complex and its connection to tumor progression is an area of active investigation

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Systemic effects of mitochondrial stress
