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The compound works through the same NNMT inhibition mechanism in both sexes, though dosing may need adjustment based on body weight

Amitai G

30 days (reconstituted) Mechanism Classification Primary Mechanism: NNMT (Nicotinamide N-methyltransferase) Inhibitor Target Enzyme: Nicotinamide N-methyltransferase Cellular Effect: Increases intracellular NAD+ and SAM levels Metabolic Pathway: NAD+ salvage pathway modulation Selectivity: Highly selective for NNMT (no cross-reactivity with related enzymes) Pharmacological Properties Route of Administration: Subcutaneous injection Bioavailability: High (subcutaneous)

R.PaigenB.NaggertJ

25 Besides the aforementioned NNMT inhibitors through a ligand-based discovery, high throughput screening has led to the identification of JBSNF-000088 ( 4 ) that inhibited hNNMT, monkey NNMT, and mouse NNMT enzymatic activities with IC 50 values of 1.8, 2.8, and 5.0 M, respectively