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Samples were mixed with 2 denaturing buffer (60 mM Tris-HCl pH 6.8, 8 M urea, 4% (w/v) SDS, 20% (v/v) glycerol, and 0.01% (w/v) bromophenol blue) in a 1:1 volume ratio, resolved by SDS-PAGE on a 10% polyacrylamide gel, and stained by Coomassie brilliant blue R-250
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for a detailed review of the kynurenine pathway and aging see van der Goot and Nollen, 2013)
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It is also worth noting that these data are definitely overestimated, as they were conducted among physicians working in specialised lipidology centres
