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FJC staining revealed extensive neuronal degeneration in peri-hematomal regions post-ICH

Add 50 L of 1 SDS-PAGE loading buffer to each tube and boil for 5 minutes

This is typically in response to hormonal changes which results in enlargement of the gland

Ans: There are mild side effects like nausea or bloating, and rare allergic reactions are possible if you take these in high doses or without supervision

Nuclear proteins are extracted from the pellet containing pure nuclear fraction, with the help of nuclear extraction buffer, which also has proteasome inhibitors to prevent nuclear protein degradation (Sundar and Tamizhselvi, 2020)