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The tissue samples were minced into small fragments, rinsed with ice-cold PBS, and homogenized using a tissue homogenizer until no visible solid particles remained (or alternatively ground in liquid nitrogen)

This was done in an attempt to extend the original background of the stomach cytoprotection concept (based on the meaningful significance of alcohol stomach lesions, induction, and prevention) [35,36], Roberts (epithelium protection) [35] and Szabos (endothelium protection) [36] intended for a large generalization