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Results GSDMD-mediated pyroptosis is activated in ischemic hindlimbs To investigate the involvement of GSDMD-mediated pyroptosis in hindlimb ischemic injury in vivo, we established hindlimb ischemia (HLI) model by ligation of the femoral artery in wild-type (WT) mice

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Transient expression of TDE0362 in HEK293T cells The TDE0362 gene sequences were codon optimized by GenScript Biotech (Piscataway, NJ)
