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Following the manufacturers instructions, 4 g of plasmid and 5 L Lipo2000 (Invitrogen) were used for transfection

doi:10.1016/j.redox.2024.103303 34
25,26 Finally, Under the action of the enzyme cystathionine--synthase (CBS), serine is coupled into homocysteine to form cystathionine, which can then be cleaved by cystathionine c-lyase (CTH) to release cysteine

doi: 10.18632/aging.103328

10.1126/science.1241459
