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Localization of Ctrs by Fluorescence Microscopy For protein localization, wild-type (WT) yeast BY4741 cells were transformed with pUG35 containing either the NgCTR or NfCTR gene, and transformants were grown overnight at 30C in liquid SC-ura medium, washed and resuspended in phosphate-buffered saline (PBS), pipetted onto a microscope slide and mixed with the same volume of 2% agarose

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Funding This work was supported by National Science Centre in Poland, grant number 2014/15/B/NZ7/00893

However, although the experimental scale of such an approach is feasible, the analysis of outcomes would be highly challenging

This method illustrates a sensible, pathogen-informed design: the parasites plentiful surface protease acts as the catalyst for payload release, guaranteeing activation mostly at the site of infection (Saeed et al., 2023