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The design of a ligand requires several steps to fulfil [2]: Determination of the binding site or possible biological interactions to use as a template for the modelling, Initial design of the ligand using this template, Preparation of a ligand library and chromatographic evaluation, Selection of the ligand of interest, Optimisation and chromatographic evaluotion of the adsorbent following the immobilization

L., Kim, S

doi: 10.1002/wnan.1958 165 JacobSKatherFSBodduSHSAttimaradMNairAB

If you used something other than BAC water (like saline), the salt concentration might be causing precipitation

2015 Nov;115(11):23572365