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Description
A 35 Ultra Semi diamond knife (Diatome) mounted on a UC7 ultramicrotome (Leica) was used to cut alternating series of sections onto formvar-coated copper mesh grids at two different thicknesses: (i) 60 nm-thick sections were visually inspected to assess ultrastructural preservation and synapse density under an 80kV LEO 912 transmission electron microscope (Zeiss), and (ii) 250 nm-thick sections were coated on both surfaces with Protein A-conjugated 10 nm gold fiducial particles (Cell Microscopy Core Products, University Medical Center Utrecht, The Netherlands) in preparation for transmission electron tomography
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What We Do Not Know We lack peer reviewed data defining systemic exposure thresholds, tissue distribution, immunogenicity rates, potential peptide related impurities impact, chronic dosing safety, interaction with existing pathologic angiogenesis (e.g
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Researchers who rely heavily on consistent, reliable communication or require rapid issue resolution may find these inconsistencies limiting
