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After incubation at 37 C for 1 h, the media were removed and formazan crystals in the cells were dissolved in 1 mL DMSO and processed for OD reading at 570 nm using a spectrophotometer

The reduction of O by electrons initially forms superoxide anion (O ), which is then either spontaneously or enzymatically converted to HO

Caution should be taken when erythromycin is co-administered with agents that alter or are metabolized by cytochrome P450 (CYP) 3A4 (e.g., diltiazem or verapamil or domperidone) because the drug interactions may be associated with risk for sudden cardiac death (Ray et al., 2004)

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Controlled storage environments between 15-25C with relative humidity below 60% optimize stability during manufacturing and finished product storage