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The assay has been made more streamlined and faster 91 , and is reproducible across laboratories 93 with pooled sensitivity and specificity for CSF alpha-synuclein SAA across 22 studies, 88% and 95% respectively 11 (Table 2)

This estimates intracellular fluxes by solving a non-linear least-squares regression problem that minimizes the variance-weighted sum of squared residuals between simulated and measured isotopic distributions of intracellular metabolites, under the assumption of pseudo-steady state (verified in Extended Data Fig

Science 2002;297:353-6

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Rabbit anti-Bax and anticaspase-3 antibodies were from Cell Signaling (Cell Signaling, Danvers, MA, USA), and rabbit anti-Bcl-2, anti-GR, and anti-GPx antibodies were from Abcam (Abcam, Cambridge, MA, UK)
