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The day after transduction, the cells were refreshed and 24h later placed on selection in media containing 0.5 g/ml puromycin

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The inserts were placed in the wells of a 24-well plate filled with 10% FBS-containing DMEM, cultured for 24 h and then stained with crystal violet (Sigma)

After the injury, BPC-157 peptide was administered intraperitoneally at doses of 10 g/kg and 10 ng/kg

"Our findings of reduced monthly rates of opioid-related toxicities, health-care use, hospital-treated infections, and non-primary-care-related health-care costs after initiation build upon earlier work showing improved clinical outcomes for people engaged with SOS programmes