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Lets say that your target protein is smaller than GST, such that most of the size of the GST-fusion protein will be contributed from GST and not your target protein

Biomed J 45(1):1118

131 Transgenic mice for superoxide dismutase-1 (SOD1), a well-known hallmark of ALS, showed NOX1/NOX2-dependent oxidative stress that has been linked to the progression of motor neuron disease

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