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After 14 days of recovery, the mice were injected with tamoxifen (TM) for 5 consecutive days, as an endeavor to stimulate active CreERT2-expressing cells and undergo Cre-mediated recombination (to be TRAPed), resulting in a permanent expression of the effector gene of mCherry

This increased training load, combined with reduced caloric intake, creates prime conditions for overuse injuries

The slides were conjugated with primary antibodies for 30 min at room temperature, followed by antibody detection using Dako REAL Envision detection system (Agilent Dako, Carpinteria, CA, USA)

(477) Zhou, F

Spheroids containing equal amounts of si CTRL transfected cells (green) and si ATG5 transfected cells were generated, embedded, and analyzed after 48 h