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Western blot assay of protein carbonylation The irradiated samples were lysed by 10 L EDTA solution (100 mM, pH 10)

If sample OD value is higher than OD value of the highest point on the standard curve, what's the suggested solution

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The concentration of the nanobody was adjusted to 1 mg/mL, and then the alkoxy group on pAcF was connected to azide group in linker (50 molar excess) through oxime ligation

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