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Description
Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

Many patients are surprised by how simple the process feels

10.1177/1535370217746612 [DOI] [PMC free article] [PubMed] [Google Scholar] Wu, Y

Available at: August 8, 2016)

High levels of drug deposited only in the lung provide active drug to the required tissues only
