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ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, USA) connected to a Waverunner LT342 digital oscilloscope (LeCroy, Chestnut Ridge, NY, USA) until the 60 min (25 mmHg), 30 min (30 mm Hg), or 30 min (40 mm Hg) reperfusion time

Discuss with provider if on metabolic medications