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Recently, this definition has been expanded to include a differentiation between the auditory phantom perception and a (related) disorder, characterized by high levels of distress 1

Insoluble material was removed by centrifugation (40,000 rpm, 60 mins) and cobalt-charged TALON metal affinity resin (~7 mL) was added to the supernatant together with 10 mM imidazole to allow batch binding for 3 hours at 4 C

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