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Dose selections for SeNPs were conducted according to previous publications with minor modifications ( Semen cryopreservation, drug treatments, and experimental group settings Before semen cryopreservation, six semen samples were individually split into 4 equals and diluted to a final concentration of 6.0 10 7 /ml spermatozoa with the basic extender supplemented with 0 g/ml SeNPs and 0 mM GSH (negative control group, NC), 2 g/ml SeNPs (SeNPs group) (50) and 4 mM GSH plus 2 g/ml SeNPs (GSH + SeNPs group)

In research settings, it is widely used to investigate hairfollicle biology, dermalcell signalling, and regenerative pathways in controlled laboratory models

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In: Advances in Surgery

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