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glutathione synthesis definition pathway Impaired Glutathione Synthesis in Neurodegeneration Glutathione synthesis. GSH synthesis relies
Description
As controls, we used Man1A1-GFP, a Golgi membrane protein with its C-terminal in the lumen (proteinase K resistant), and CTNS-GFP, a lysosome membrane protein with the C-terminus facing the cytosol (proteinase K sensitive)
The protein concentration was determined using a BCA Protein Assay Kit (KeyGEN Bio TECH, Nanjing, China)

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