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therefore, we quantified the expression level of this gene after epitalon treatment

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the latter is bioactive, bypassing conversion needs

Subsequently, the binding affinity of native semaglutide and GLP-1R ECD was calculated by Prodigy [30, 31] to be 3.4 10 6 M at 37 C, with the template being PDB entry: 4ZGM [24], where a total of 42 residue pairs were found to be located at the binding interface of semaglutide and GLP-1R ECD [24, 35], as listed in Table 4 of supplementary file supps.pdf