glutathione cancer review Glutathione-mediated nanomedicines for diagnosis and therapy ACTL6A protects gastric cancer cells

Next, two cell lines, DLD-1 (KRAS G13D/- ) and HT29 (BRAF V600E ), were used as models of intrinsic resistance to cetuximab, and Caco-2-CR was used as a model of acquired resistance to cetuximab for further study

All experiments were performed at 25 C in a buffer solution containing 20 mM HEPES (pH 7.0), 150 mM NaCl, and 1 mM DTT

All clamp experiments were performed at the National Mouse Metabolic Phenotyping Center at UMass Medical School

Presence or absence of metal ions in the reaction mixture did not affect the enzyme activity

Then FcRn-GST (0.25 g/ml or 2.0 g/mL), biotinylated human or mouse FcRn (Immunitrack) (2.5 g/mL or 0.25 g/mL) were added and incubated for 1 h at RT