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The data, together with the data from the same study measuring pyruvate levels described above, indicate that fasting increased the fumarate/pyruvate, and so also likely increased the malate/pyruvate, indicative of the [NADPH]/[NADP + ], in the spinal cord and several brain regions, including the hippocampus, brainstem, olfactory bulb, and potentially to a smaller extent in the cortex, while potentially decreasing in the hypothalamus (Shao et al., 2023)

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We stress that those methods, even when used as unsupervised learning techniques, often have hyperparameters and settings to tune, and they should also be validated on external data not used during the initial analysis

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This matters for those planning families or concerned about hormonal effects over time