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siRNAs and shRNA plasmids To generate constitutive knockdown cell lines, plasmid DNA was purified from pLKO.1 backbone vectors expressing shRNAs targeting DDX3X, CBS, GCLC, METTL16 and a scrambled control
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For example, RASSF1A, a key node linking the Hippo and RAS signaling pathways, frequently undergoes promoter hypermethylation in renal cell carcinoma and lung cancer [84, 85]
Corvo ML, Boerman OC, Oyen WJG, Bloois LV, Cruz MEM, Crommelin DJA, et al
Crit Rev Microbiol 45:131161
