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Even though high-speed high-resolution mass spectrometers, such as the one used in this work, have the ability to acquire spectra very fast and with high sensitivity, there are still many peptides not triggered for MS/MS acquisition under these conditions

FIGURE 1 2 Mitochondria and reactive oxygen species 2.1 ROS generation ROS, such as superoxide anions, hydrogen peroxide, and hydroxyl radicals, are reactive molecules containing oxygen and are mainly generated in the mitochondria (Shrestha et al., 2021)

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