glutathione defficiency deficiency alters the vitamin D-metabolizing enzymes CYP27B1 and CYP24A1 in human renal proximal tubule epithelial cells and kidney of HFD-fed mice Glutathione deficiency: what to do
Description
To identify the exact mutations in ABCC1 knockout clones, PCR products were subcloned using the TA Cloning Kit (Thermo Fisher Scientific, USA) and two clones were analyzed by Sanger Sequencing
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The raw MS and MS/MS spectra were processed using the Proteome Discoverer software by Thermo Scientific (v1.4) and searched against the Uniprot human database using the SequestHT search algorithm
Cramton, Sarah E
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