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Heres the complete protocol we recommend at SL Aesthetics Clinic: 1
Notably, an autophagy-dependent FRG coprediction model suggested that the expression of SIRT1 may be associated with macrophage infiltration and immune tolerance in glioma tissues (136)

doi: 10.1016/j.ifset.2015.11.017

Immunofluorescence Cells grown on coverslips were fixed with cold methanol at 20 C and then incubated with the following primary antibodies: anti-LC3A/B rabbit monoclonal (1:100) (Cell Signaling Technology, Inc., New England Biolabs., UK), anti-Phospho-S6 Ribosomal Protein rabbit monoclonal (1:100) (Cell Signaling Technology), anti p53 mouse monoclonal (1:100) (Dako Cytomation, Glostrup, Denmark) and anti-Ki67 rabbit polyclonal (1:300) (Abcam, Cambridge Science Park, Cambridge, UK)

FBS was purchased from PAA Laboratories (Austria)
