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The reaction is then stopped using a stop solution provided in the kit

Then, the cells were trypsinised, fixed with 4% PFA, permeabilised and labelled with Alexa Fluor 647 picolyl azide

This increase was discussed in thread below
Superoxide radical production in chicken skeletal muscle induced by acute heat stress

The hypomethylation of DNA, a marker of early carcinogenesis, is another effect of the B12 lack
