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200 L standard solution was mixed with 100 L sodium carbonate buffer (0.1 M, pH = 9.5), 100 L 2% BzCl and 1 L formic acid at room temperature for 5 min

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According to other research, small cohorts of studies have found 1 Mb deletion in the 5q14.3 region [93], which resulted in a significant reduction of complex IV activity, and complex I activity was only slightly reduced

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