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Phage was overlaid on the sections at room temperature for 30 min, and sections were then washed with PBS and fixed with 1% paraformaldehyde (PFA) in PBS

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Lipotropics are nutrients involved in hepatic lipid handling and methyl-group transfer (for example, choline and methionine )

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It was originally identified as a fragment derived from a larger protein found in human gastric juice, which sparked early scientific interest due to its unusual stability and biological activity under experimental conditions