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The max , used for the absorbance measurements are 515 nm (Gomez-Alonso et al

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doi: 10.1016/j.bbadis.2013.10.007
For transient transfection, HT22 mouse hippocampal cells were seeded in 6-well plates at an approximate confluency of 1.0 10 6 cells/mL and then cultured for 24 h in DMEM with low glucose containing 10% fetal bovine serum, 2 mM L-glutamine, and penicillin (100 units/mL)/streptomycin (100 g/mL)

Toxicol Appl Pharmacol [Content Brief] [3]
