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In two patients, empty sella was detected on MRI, but this finding was considered a normal variation

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The conditioned media were collected after 12 h incubation, centrifuged at 1000 g for 10 min, filtered through a Nalgene 0.2 m pore vacuum filter, and concentrated using a 10 kDa MWCO Amicon Ultra-15 centrifugal filter (MiliporeSigma) operating at 1500 g for 30 min with 50 mM ammonium bicarbonate buffer until the final concentration was 2 mg/ml

(2023) Metabolomic and lipidomic signatures in autosomal dominant and late-onset Alzheimers disease brains

In addition to these environmental factors, other population differences, such as linkage disequilibrium of the GPx-1 locus with other specific modifying genes, the influence of other gene variants that act to modulate GPx-1 activity [such as the SelP subtypes mentioned above (252)], and/or the presence of other gene variants that act in concert with GPx-1 to modify disease risk may also explain differences among studies performed in different populations